Nucleotide Sugar Transport
The delivery of nucleotide sugar substrates into the endomembrane for processes such as cell wall biosynthesis and protein glycosylation is critical for plant growth and development. Although plant genomes encode large families of putative nucleotide sugar transporters (NSTs) that deliver the diverse array of nucleotide sugars found in plants, few have been characterized. Recently, we developed a yeast proteoliposome transporter assay coupled to LC-MS, specifically designed to characterize NSTs.
- Molecular characterization of the Arabidopsis Golgi-localized UDP-GlcNAc transporter
- Biochemical characterization of UDP-Arap transporter from Arabidopsis
- Biochemical characterization of UDP-GlcA and UDP-GalA transporters from Arabidopsis
- Identification of the PAPS transporter in Aspergillus
- Informatic and biochemical survey of the NST family in rice