Subcellular Proteomics
Much of my groups research focuses on the utilization of subcellular proteomic data from compartments involved in plant cell wall biosynthesis (e.g. Golgi apparatus, cytosol and endoplasmic reticulum) to investigate protein function and metabolic partitioning. The investigation of these processes has resulted in the development of novel tools, strategies and basic knowledge that can be used to better understand cell wall biosynthesis in plants.
Current Projects
- Isolation and proteomics characterization of the Golgi and sub-Golgi from Arabidopsis by optimized free-flow electrophoresis
- Comparative analysis of the Arabidopsis Golgi proteome after treatment with inhibitors e.g. brefeldin A or cycloheximide
- Comparative analysis of the Arabidopsis Golgi proteome after manipulation of cell wall composition by the exogenous addition of monosaccharides.
